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KMID : 0903520050480030228
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 3 p.228 ~ p.232
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Production and Reaction Properties of Phytase by Saccharomyces cerevisiae CY strain
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Seo Sung-Won
In Man-Jin
Oh Nam-Soon
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Abstract
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A yeast strain producing phytase, isolated from a mash of Korean traditional Yakju, was identified as a strain of Saccharomyces cerevisiae and designated as Saccharomyces cerevisiae CY strain. Phytase was produced by CY strain both intracellularly and extracellularly. Total phytase activity by the shaking culture was about two times higher than that of the static culture. The portion of extracellular phytase to total phytase activity ranged between 23 and 49 percent, depending on the glucose concentration in the culture medium. Phytase production was reached at approximately 1 U/ml as total phytase activity and the maximum intracellular phytase activity was 0.17-0.19 U/mg-DCW at late logarithmic growth phase. The optimum reaction pH and temperature of intracellular phytase were 3.5 and 40oC, respectively. Over 95% of the phytate was degraded by growing cells after 36 hours yeast cell culture and about 90% of total phytate was effectively degraded by suspending the whole cell with the biomass of 0.4 mg-DCW/ml-reaction solution after 12 hours degradation reaction.
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KEYWORD
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Saccharomyces cerevisiae, phytase, phytate degradation
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